<strike id="w0d4m"></strike>
<ins id="w0d4m"><b id="w0d4m"></b></ins>

      <legend id="w0d4m"><strong id="w0d4m"></strong></legend>

      AAV9 Titration ELISA Test Kit

      AAV9 Titration ELISA Test Kit is a competitive enzyme immunoassay for the quantitative determination of AAV serotype 9 particles in cell culture supernatants and purified virus preparations.

      • 產(chǎn)品品牌: REAGEN
      • 產(chǎn)品貨號: RCGT09

      Product Description

      REAGEN AAV9 Titration ELISA Test Kit is a competitive enzyme immunoassay for the quantitative determination of AAV serotype 9 particles in cell culture supernatants and purified virus preparations.

      Adeno-associated viruses (AAV) are non-pathogenic ssDNA viruses, which are subject of intense studies as viral vectors for gene therapy. The virus transduces a variety of dividing and non-dividing cells showing long term gene expression with low cellular immune response. AAV has been used in several clinical trials (e.g. FIX, CFTR, Parkinson's, Canavan disease) showing no serious vector-related adverse effects. Methods for the characterization of AAV preparations currently include titration ELISA, qPCR, ddPCR, DNA dot blot, determination of transducing units, infectious center assay, SDS-PAGE or electron microscopy. Immunotitration by REAGEN' AAV9 Titration ELISA offers a fast, sensitive and reproducible method for titration of intact AAV9 wild-type virions, AAV9 recombinant virions or assembled and intact empty AAV9 capsids.

       

      Procedure Overview

      REAGEN The assay is based on the sandwich ELISA technique. A monoclonal antibody specific for a conformational epitope on assembled AAV9 capsids is coated onto strips of a microtiter plate, captured AAV particles are detected in two steps:

      1. Another Anti-AAV9 antibody was used as detection antibodies.

      2. A Anti-Human IgG Fc Secondary Antibody reacts with the immunocomplex.

      Addition of substrate solution results in a color reaction, which is proportional to the amount of specifically bound viral particles. The absorbance is measured photometrically at 450 nm (optional: reference wavelength at 620 nm).

      The provided AAV9 standard contains an AAV9 particle preparation of empty capsids. Two-fold serial

      dilutions of the material result in a typical titration curve. The curve allows the quantitative determination of samples of an unknown particle titer.

      .

      Kit Contents, Storage and Shelf Life


      AAV9 Titration ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C . The shelf life is 12 months when the kit is properly stored.

      Kit Contents

      Amount

      Storage

      Coated Plate

      1 x 96-well Plate

      (8 wells x 12 strips)

      2-8°C

      AAV9 Standard, lyophilized

      3

      2-8°C *

      Anti-AAV9 Antibody

      12 mL

      2-8°C *

      Anti-Human IgG Fc Conjugate

      12 mL

      20X Assay Buffer

      50 mL

      Stop Buffer

      7 mL

      TMB Substrate

      12 mL


      企業(yè)愿景

        公司將一如既往的視誠信為最重要的文化精神;在經(jīng)營決策和管理的全過程中信守“以產(chǎn)品質(zhì)量、優(yōu)質(zhì)服務”為原則和“為人類健康服務”為宗旨的理念;融前沿科技、全球資源與優(yōu)質(zhì)人才為資本,以開拓創(chuàng)新、銳意進取、精益求精之精神,科學管理之導向,凝聚員工、客商之合力,同舟共濟,為實現(xiàn)人類的食品安全和生物醫(yī)藥業(yè)的高速發(fā)展不懈奮斗!

      查看更多

      工作時間

      客戶熱線: 0755-2300-6699
      工作時間: 09:00AM-20:00PM一流的專業(yè) 一流的服務
      亚洲国产精品精品在线电影,99在线成人精品视频,亚洲中文字幕永久网站,欧美三级欧美一级在线视频
      <strike id="w0d4m"></strike>
      <ins id="w0d4m"><b id="w0d4m"></b></ins>

        <legend id="w0d4m"><strong id="w0d4m"></strong></legend>